| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas
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| Autores: |
Corporeau, Charlotte
Groisillier, Agnes
Jeudy, Alexandra
Barbeyron, Tristan
Fleury, Elodie
Fabioux, Caroline
Czjzek, Mirjam
Huvet, Arnaud
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| Data: |
2011-10
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| Ano: |
2011
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| Palavras-chave: |
Transforming growth factor-beta
Bacterial expression
In vivo antibody inhibition
Reproduction
Crassostrea gigas
Marine bivalve
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| Resumo: |
The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-beta protein, and we demonstrate that og TGF-beta is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-beta in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-beta function tends to reduce gonadic area. We conclude that mature og TGF-beta probably functions as an activator of germ cells development in oyster.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00042/15371/13438.pdf
DOI:10.1007/s10126-010-9361-4
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| Editor: |
Springer
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| Relação: |
http://archimer.ifremer.fr/doc/00042/15371/
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| Formato: |
application/pdf
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| Fonte: |
Marine Biotechnology (1436-2228) (Springer), 2011-10 , Vol. 13 , N. 5 , P. 971-980
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| Direitos: |
Springer Science+Business Media, LLC 2011
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